Search results for "V79 cell"
showing 4 items of 4 documents
Molecular studies on the toxifying effects by genetically engineered cytochromes P450.
1999
After almost two decades, it is now evident that methodology based on molecular biology and gene technology has dramatically changed the way basic and applied toxicology is being performed. It star...
Glutathione content of V79 cells in two- or three-dimensional culture
1997
The cellular glutathione (GSH) content of two- and three-dimensional cell cultures of V79 hamster lung cells has been studied. As previously described, cells in monolayer cultures show a decrease in GSH when they reach the confluent state. Three-dimensional cell cultures (multicell spheroids) allow a smoother transition from the initial proliferating to the nonproliferating status, and they show a central area of necrosis when a certain diameter is reached. Cellular GSH content in spheroids is variable throughout the culturing period: 1) GSH content (expressed per mg protein) is lower in spheroids with central necrotic areas than in smaller spheroids without necrosis, and 2) results expres…
Acrylamide catalytically inhibits topoisomerase II in V79 cells.
2010
The vinyl monomer acrylamide is characterized by the presence of an alpha,beta-unsaturated carbonyl group that makes it reactive towards thiol, hydroxyl or amino groups and towards the nucleophilic centers in DNA. The ability of acrylamide to chemically modify protein thiols has prompted us to consider topoisomerase II as one possible target of acrylamide, since agents targeting protein sulfhydryl groups act as either catalytic inhibitors or poisons of topoisomerase II. Nuclear extracts from V79 Chinese hamster cells incubated with acrylamide reduced topoisomerase II activity as inferred by an inability to convert kinetoplast DNA to the decatenated form. Nuclear extracts incubated with acry…
Biological effects of alpha-pinene in cultured mammalian cells
2009
In this work we report the effects of exposure of mammalian cells to α-pinene, a bicyclic monoterpene founded in essential oils and used in insecticides, solvents, perfumes, etc.. Morphological analysis, performed in V79 cells exposed to increasing doses(25μM up to 50μM) of α-pinene, indicated a increase of dose-related nuclear abnormalities; apoptotic cells were seen at higher doses. Immunofluorescence with anti β- tubulin antibody showed that monoterpene induced genomic instability by interfering with mitotic process; in fact, 50% (vs 19% in the control cells) of irregular mitosis with multipolar or not correctly localized spindles were detected, suggesting that α-pinene affects cell stab…